Western blot analysis of extracts from L929 cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® TNF-R1 siRNA I (Mouse Specific) #13369 (+) or SignalSilence ® TNF-R1 siRNA II (Mouse Specific) (+), using TNF-R1 (D3I7K) Rabbit mAb (Rodent Specific) #13377 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The TNF-R1 (D3I7K) Rabbit mAb (Rodent Specific) confirms silencing of TNF-R1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.